An ON-type direction selective ganglion cell in primate retina

To maintain a stable and clear image of the world, our eyes reflexively follow the direction in which a visual scene is moving. Such gaze stabilisation mechanisms reduce image blur as we move in the environment. In non-primate mammals, this behaviour is initiated by retinal output neurons called ON-type direction-selective ganglion cells (ON-DSGCs), which detect the direction of image motion and transmit signals to brainstem nuclei that drive compensatory eye movements. However, in primates, ON-DSGCs have not yet been identified in the retina, raising the possibility that this reflex is mediated by cortical visual areas. Here, we mined single-cell RNA transcriptomic data from primate retina to identify a candidate ON-DSGC. We then combined two-photon calcium imaging, molecular identification, and morphological analysis, to reveal a population of ON-DSGCs in the macaque retina. The morphology, molecular signature, and GABAergic mechanisms that underlie direction selectivity in primate ON..., , , # An ON-type direction selective ganglion cell in primate retina

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This dataset contains the raw data from the paper: \"An ON-type direction selective ganglion cell in primate retina\".

The data are from the following experiments:

* Immunohistochemistry (IMHC): Pieces of macaque retina were stained for BNC2, RBPMS, and FOXP2 to analyze the density and proportion of pRGC10s and pRGC16s (Figure 1d-e), and their mosaic properties (Extended Data Fig. 1). IMHC was performed after calcium imaging to determine the molecular identity of recorded cells (macaque; Figure 2, mouse; Extended Data Fig. 5.). Candidate human On-DSGCs were labeled with BNC2 and RPBMS (Figure 4). Additional antibody validation was also performed (Extended Data Fig. 7).
* Calcium imaging: Calcium responses to light stimuli were recorded from the ganglion cell layer in ex vivo macaque (Figure 2, Figure 3e-h) and mouse retinas (Extended Data Fig. 5). Stimuli include a bar drifting in 8 directions (2.24 degrees/s for mac...