Effects of livestock manure properties and temperature on the methanogen community composition and methane production during storage

Livestock slurry stored in ponds is an important source of methane emission, which is influenced by environmental factors. In this study, the effect of slurry properties and temperature on methane flux and methanogen community composition was investigated. The methanogen community composition in swine slurry was more sensitive to temperature and significantly different from that of cattle slurry (ANOSIM, P Methanobrevibacter, Methanocorpusculaceae and Methanocorpusculum. These different methanogen communities partially accounted for the differences in methane flux between swine and cattle slurries. Methanogen abundance seemed to not be affected by slurry properties or temperature, but the mcrA (encoding the alpha subunit of methyl coenzyme M reductase) transcript/gene ratio was significantly increased at 30°C and was higher in swine slurry than in cattle slurry (t-test, P mcrA rather than by increasing methanogen cell numbers.