Regional Conservation and Transcriptional Regulation of Tumor-Associated Genes by macroH2A1 Deposition in Mammalian Cells

Histone variant macroH2A1 has been widely recognized as a suppressor of gene expression. Recently, a cell cycle dependent deposition of macroH2A1 is discovered in mouse cells, but whether this process exists in human chromatin is unclear, which might be crutial for related diseases, particularly cancer. In this study, we firstly demonstrate that dynamic macroH2A1 domains occurs in both normal and cancerous human cells. Our findings provide new epigenetic insights into the role of macroH2A1 in malignant proliferation, offering a novel perspective for furture cancer research. native Chromatin immunoprecipitation and DNA-sequencing (nChIP-seq) for histone variants macroH2A1 in ARPE-19 cells and HeLa cells in G1/S phase and metaphase