Proteomic Analysis of Soybean Root Tissues Before and After Inoculation with Heterodera glycines (Soybean Cyst Nematode)

This project aims to investigate the proteomic responses of roots from soybean (Glycine max) inoculated with soybean cyst nematode (Heterodera glycines, SCN) versus non-inoculated (pre-inoculation) soybean roots as control. Soybean root samples were collected from both groups and subjected to quantitative proteomic analysis using LC-MS/MS-based techniques, including protein extraction, tryptic digestion, and [specify quantification method, e.g., label-free quantification or TMT-based quantification]. Differentially expressed proteins were identified to characterize molecular mechanisms underlying soybean root responses to SCN infection.