rCRUX Generated MiSebastes Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022.

Primer Name:  MiSebastes
Gene:   CytB
Length of Target:    153
get_seeds_local() minimum length:    107
get_seeds_local() maximum length:    199
blast_seeds() minimum length:    71
blast_seeds() maximum length:    163
max_to_blast:  100
Forward Sequence (5'-3'):   AAGCTCATTCAAGTGCTT
Reverse Sequence (5'-3'):    GACCACTTACACAATTCT
Reference:   Min, M. A., Barber, P. H., & Gold, Z. (2021). MiSebastes: An eDNA metabarcoding primer set for rockfishes (genus Sebastes). Conservation Genetics Resources, 13(4), 447-456. https://doi.org/10.1007/s12686-021-01219-2

We chose default rCRUX parameters for get_blast_seeds() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for blast_seeds() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.