Comparison of in vitro and in vivo DNA binding experiments for all four Zur promoters.

In vitro analyses were measured by EMSA gel-shift experiments and in vivo experiments were performed using RT-PCR experiments monitoring the fold change in mRNA production in between both WT and Δzur conditions. Δ(ΔG°) was calculated using the Kd-app value for each promoter and comparing the free energy to the weakest binder PpliG. This table demonstrates that both in vivo and in vitro data support the Zur hierarchy of affinity as follows: PL31P and PzinT>>PznuC and PpliG. See Data S10 for the raw data used to generate each value.a6 pM [DNA] was used to ensure sub-stoichiometric titrations of DNA were added to the protein-DNA samples.Comparison of in vitro and in vivo DNA binding experiments for all four Zur promoters.