Skeletal muscle regeneration is compromised in advanced diabetic peripheral neuropathy

DPN muscle exhibits features of degeneration with attempted regeneration. In the most severely pathological muscle samples, regeneration appears to be stymied and our data suggest that this may be partly due to intrinsic dysfunction of the satellite cell pool in addition to extrinsic structural pathology (e.g. nerve damage). Biopsies were acquired from two muscles in individuals with and without diabetic neuropathy undergoing below knee amputation surgery. Biopsies were subdivided for histological analysis, transcriptional profiling and satellite cell isolation and culture. DPN: diabetic peripheral neuropathy MNC: metabolically normal control