Ischemia reperfusion injury (IRI) following syngeneic lung transplantation

Ischemia reperfusion injury (IRI) continues to be an important factor contributing to long-term outcomes after transplantation. Here, utilizing a murine model of orthotopic left lung transplantation, we collected single cell RNA sequencing data from naive lungs and syngeneic lung grafts at various time points up to 30 days. Overall design: We performed syngeneic orthotopic murine left lung transplants from CD45.2+ C57BL/6J to CD45.1+ C57BL/6J mice with 60 minutes of cold ischemia at 4°C then 45 minutes of warm ischemia at 28°C. Lung grafts were collected at various time points after reperfusion including 2 hours (WT2H), 3 days (WT3D), 7 days (WT7D), and 30 days (WT30D). Additionally, we collected single cell suspensions from naïve C57BL/6J left lungs (CTRL). For each sample (CTRL, WT2H, WT3D, WT7D, WT30D), we pooled cells from two lungs and sorted recipient CD45.1+ cells, donor CD45.2+ cells, and stromal CD45.1- cells. We then performed single-cell RNA sequencing on these samples.