Effects of excluding protein constituents on in vitro CpI activation.
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Except for the protein constituents indicated under Condition (Δ), reaction mixtures contained a ΔiscR E. coli lysate (either clarified or desalted), 5 µM HydE, 5 µM HydF, 50 µM HydG, 1 µM CpI, and the standard set of extrinsic substrates. Either no NTP or ATP (15 mM) was added instead of GTP (15 mM) for selected conditions.†CpI specific activities are shown in units of µmol H2 consumed·min–1 mg CpI–1 (n = 4–8).
创建时间：
2015-12-02
