CD14hi/lo M1 and M2 MΦ cytokines are differentially tolerised by P.gingivalis and LTA PAMPs.

CD14hi/lo M1 and M2 MΦ subsets exhibit a differential tolerisation and cross-tolerisation of cytokine production to the P gingivalis PAMPs, PG-LPS and HKPG as well as the TLR2 PAMP, lipoteichoic acid (LTA). CD14lo M1, CD14hi M1, CD14lo M2 and CD14hi M2 MΦ subsets were pre-stimulated (tolerised) with either 100 ng/ml PG-LPS, 1×107 cells/ml HKPG or 10 µg/ml LTA for 24 hours prior to stimulation with PG-LPS, HKPG or LTA and incubated for a further 18 hours. Tolerisation/suppression of the pro-inflammatory cytokines, TNFα (also refer to graphs in figure 6), IL-1β and IL-6 is expressed as the mean percentage suppression ± SD of non-tolerised stimulation controls. Data displayed represents triplicate samples for n = 3 replicate experiments.