Metagenomic Insights into PVC Microplastic Degradation by Kosakonia pseudosacchari FR-4 in Simulated Aquaculture Biofilms

Microplastic pollution in aquaculture environments poses significant ecological risks, including biofilm formation on plastic surfaces that harbors antibiotic resistance genes (ARGs) and quorum sensing (QS) systems, potentially disrupting microbial communities. This BioProject presents metagenomic sequencing data from a novel study isolating and applying a PVC-degrading bacterium, Kosakonia pseudosacchari FR-4, derived from field aquaculture sediments, to mitigate these impacts.Experimental Design: A simulated aquaculture pond water model was constructed using sterile pond water inoculated with PVC microplastics (50-100 um particles; n=3 replicates per treatment). Quartz sand served as a non-plastic blank control. K. pseudosacchari FR-4 was introduced at three timings: (1) early phase (Day 0, pre-biofilm), (2) mid-phase (Day 14, post-biofilm establishment), and (3) no addition control. Incubations lasted 28 days at 25C with gentle aeration to mimic real conditions. Water and microplastic-associated biofilms were sampled at Days 0, 7, 14, 21, and 28 for total DNA extraction.Data Generated: High-throughput Illumina NovaSeq metagenomic reads (paired-end, ~150 bp) from 45 samples (15 timepoints x 3 treatments), totaling ~X Gb (to be specified post-upload). These datasets enable quantification of K. pseudosacchari FR-4's degradation efficiency on PVC (via plastisphere shifts), alterations in biofilm bacterial communities (alpha/beta-diversity, keystone taxa), prevalence of ARGs and QS genes (e.g., luxS, rhlI), and overall microbial resilience in aquaculture settings.Significance: This resource advances understanding of bio-based plastic remediation in high-density farming systems, informing sustainable practices to reduce microplastic-driven AMR dissemination. Raw reads are deposited in SRA under SUB15829194; processed data (assemblies, annotations) available upon request.