Next generation sequencing facilitates quantitative analysis of transcriptional profiling of Candida albicans wild type

Illumina NovaSeq 6000 was used to deeply sequenced the mRNA expression of the AMB treated group, the AMB and Rimonabant combined treatment group and the vehicle-treated Candida albicans SC5314, and three repeated mRNA expressions were obtained. The analysis was performed at the isoform level by sequence reading through a quality filter. SYBR Green method was used for QRT-PCR validation. We found that after Amb treatment, the regulatory genes MDM12, PSD2, OPI3, INO2 and PMP3 related to lipid synthesis were up-regulated, and INO2 and MDM12 were significantly down-regulated after combined treatment.Some genes involved in lipid modification and oxidative phosphorylation also changed accordingly mRNA Profiles of Candida albicans SC5314 processed by Amb，Amb and Rimonabant,and vehicle-treated were obtained by deep sequencing.