The age of chick embryos (days) and the number of successful replicates per number of attempts.

Each replicate used one embryonic chicken and 6 culture attempts to form agglomerates were set up in each case. The formation of at least 5 out of 6 agglomerates was required for the scoring as successful. When 19 and 20 day chicks were used, agglomerates were not formed. Each replicate used one embryonic chicken, and six mini organs were attempted for each replicate. At least 5 out of six mini organs must be formed for the attempt to count as a successful replicate. For day 19 and 20, none of the mini organs was successfully formed in all three replicates.