Early life exposure to environmental contaminants (BDE-47, TBBPA, and BPS) produced persistent gut dysbiosis in adult male mice

The gut microbiome is a pivotal player in toxicological responses.  We investigated the effects of maternal exposure to 3 human health-relevant toxicants (BDE-47, TBBPA, and BPS) on the composition and metabolite levels (bile acids [BAs] and short chain fatty acids [SCFAs]) of the gut microbiome in adult pups.  CD-1 mouse dams were orally exposed to vehicle (corn oil, 10ml/kg), BDE-47 (0.2 mg/kg), TBBPA (0.2 mg/kg), or BPS (0.2 mg/kg) once daily from gestational day 8 to the end of lactation (postnatal day 21). 16S rRNA sequencing and targeted metabolomics were performed in fecal DNA of 12-week-old adult male pups (n=14-23/group).  BPS had the most prominent effect on the beta-diversity of the fecal microbiome compared to TBPPA and BDE-47 (QIIME).  Seventy-three taxa were persistently altered by at least 1 chemical, and 12 taxa were commonly regulated by all chemicals (most of which were from the Clostridia class and were decreased).  The most distinct microbial biomarkers were S24-7 fo...