<i>In silico</i> (BLAST) search for primer sequences from <i>F. virguliforme</i> in the IGS sequences of other <i>Fusarium</i> spp. and other fungi expected in fields with corn and soybean production.

aA GenBank number was provided if the search provided a reference. bThe blast search was conducted if a GenBank entry was found. The highest value of the maximum score is given. “(Y)” The 3′ end of the forward and reverse primers at least matches 5 bases of the shown sequences separately, which means the possible success of amplification for the sequence in the database by Fv-IGS primer. “(N)” The 3′ end of the forward and reverse primers does not match at least 5 bases of the shown sequences separately, which means very low possibility of successful amplification for the sequence in the database by Fv-IGS primer. “(NN)” The possible amplification size is too big (>5000 bp). cThe hit sequences were in IGS region. dThe hit sequences were not in IGS region, and they were in other nuclear regions. “N/A” not applicable because no matches were deposited in GenBank.