scRNA-Seq of immune populations from nasal mucosa and lungs of C57BL/6 mice and BLT1 receptor deficient mice infected intranasally with MCMV K181 strain

We used a single cell sequencing approach using 10x Genomics scRNAseq to understand the transcriptional differences between immune populations from MCMV infected wildtype C57BL/6 mice and BLT1-/- mice. IFNy producing cells were similar between both experimental groups, however, BLT1-/- mice showed a reduced contribution of IFNy from CD8+T cells which was sublte in the nasal mucosa (NM) but obvious in the lungs. Comparison of T cell clusters showed that WT T cells had increased gene signatures associated with proliferation, integrin signaling, cytoskeleton regulation by Rho GTPases, T cell activation, and cytokine/chemokine signaling compared to BLT1-/- T cells, with this being true in CD8 clusters in the NM and both Cd4 and CD8 in the lungs. Overall design: Following MCMV intranasal infection, immune cells from nasal mucosa and lungs were isolated by fluorescence-acitivated cell sorting (FACS) and analyzed using scRNA seq and CITEseq.