RNA-seq of E. coli strains overproducing DNA \"damage-up\" proteins (DDPs)

This experiment assesses gene expression changes in response to the overproduction of DNA \"damage-up\" proteins (DDPs). These proteins, when overproduced, activate the SOS DNA damage response and were identified in a genome-wide overproduction screen using the mobile plasmid collection (Saka et al 2005). For the RNA-seq experiments, 7 DDP overproduction strains were compared to an empty vector control strain.