Supplementary Material

The supplementary material provides an overview of methodologies and results related to acoustofluidic cell separation. It includes sections detailing the simulation approach and a sensitivity analysis that highlights how design parameters affect response factors such as cell recovery rate and energy consumption. Schematic representations of the MOPSO and DE algorithms formulation illustrate their components and interactions. Additionally, the detailed fabrication process of the acousto-microfluidic chip is described, along with data-driven surrogate modeling data-sheets. The design nodes and their corresponding response values presented, while results from MOPSO and DE solutions are also included, offering insights into machine learning optimization outcomes.