Genome-wide CRISPR knockout library screening coupled with high-throughput sequencing to analyze DNA repair outcomes

Cells were transduced with a genome-wide CRISPR knockout library to introduce gene disruptions across the genome. After Cas9-induced DNA double-strand breaks, repair outcomes were captured and quantified using high-throughput sequencing. This experiment aims to identify genetic factors influencing specific DNA repair outcome patterns such as 1-bp insertions, microhomology-mediated deletions, and large insertions.