[E-MTAB-434] Transcription profiling by high throughput sequencing of zebra fish tissues and developmental stages. Danio rerio

Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages using the Illumina Genome Analyzer Paired-end sequence data has been generated using polyA selected RNA from a range of zebrafish tissues and developmental stages using the Illumina Genome Analyzer. These data have been used to improve the gene annotation of the zebrafish genome. Study description: Zebrafish total RNA was extracted from embryonic and adult tissue, then polyA selected. After fragmentation and reverse transcription Illumina sequencing libraries were prepared. Paired-end sequence runs were performed with 36, 37, 54 and 76 base reads on the Illumina Genome Analyzer. ArrayExpress Release Date: 2011-03-11 Person Roles: submitter Person Last Name: Collins Person First Name: John Person Mid Initials: E Person Email: [e-mail] Person Phone: [phone] Person Address: Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1HH, United Kingdom Person Affiliation: Wellcome Trust Sanger Institute Overall design: Experimental Design: development_or_differentiation_design Experimental Factor Name: AGE Experimental Factor Name: DEVELPOMENTAL_STAGE Experimental Factor Name: ORGANISM_PART Experimental Factor Type: age Experimental Factor Type: developmental_stage Experimental Factor Type: organism_part