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Sequences that influence mRNA trans splicing and polyadenylation in Trypanosoma brucei

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https://www.ncbi.nlm.nih.gov/sra/ERP137045
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资源简介:
Trypanosoma brucei library consisting of a pool of reporters whose 14Ts polypyrimidine tract is replaced with 11 random nucleotides. The library is treated with puromycin at a concentration of either 0.2ug/ml, 0.4ug/ml or 1ug/ml. The reporters are recovered and PCR amplicons targeting the random sequences identified by HTS. The results are paired reads, where the suffixes _1 and _2 are reads 1 and 2 respectively. Also included are sequencing results from the plasmid library.
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2023-10-13
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