Summary of ligand binding properties of wild type TP and mutants*.

*The values are expressed as the mean ± S.E (n = 2 to 5 experiments in duplicate), and the experiments are performed using the TP antagonist [3H] SQ 29,548 as the radioligand (NET936250UC, PerklinElmer).┼No significant specific binding to [3H] SQ 29,548 detected for these mutant receptors under the assay conditions.1Kd, Affinity of the antagonist SQ 29,548 for the receptor.2Bmax, Binding maximum of the ligand SQ 29,548 for the receptor. Usually expressed as picomoles of TP per milligram of total membrane protein.3EC50 the molar concentration of agonist U46619 that produces 50% of the maximal possible effect (calcium mobilized) for TP and mutant receptors.