I?B? is a key transcriptional regulator of IL-36-driven psoriasis-related gene expression in keratinocytes

Human primary keratinocytes were lentivirally transfected with control or NFKBIZ shRNA followed by stimulation with 1.5 and 24 h with IL-36?. Total RNA was extracted using TRIZOL. After mRNA purification with the NEBNext mRNA Isolation Kit, NEBNext Ultra RNA Library Preparation Kit was used for library construction.