CD96+ and CD96- NK cells from human livers. Homo sapiens

To better define primary CD96+ NK cells and CD96- NK cells from human liver, we isolated primary hepatic lymphocytes from healthy liver and purified CD96+ and CD96- NK cells through negative selection and flow cytometry sorting. Purified NK cells from human liver tissues were first enriched by MACS using NK Cell Isolation Kit (Miltenyi Biotec, German) and CD96+/- hepatic NK cells were isolated by FACS Aria cell sorter (BD Biosciences, United States) to attain a purity greater than 95%. Overall design: Expression profiling of CD96+ hepatic NK and CD96- hepatic NK cell types. Three healthy donors were pooled for each cell type.