Using the tiller bases of wheat variety KN199 and its CRISPR/Cas9-based TaLA1 edited lines as materials, RNA was extracted and subjected to transcriptome sequencing.
Single cell RNA sequencing (scRNA-seq) was used to find the key potassium channels in insulin secretion. Overall design: We used a system involving CRISPRâCas9-mediated knockout of all 83 K+ channel
