TusA influences Fe-S cluster assembly and iron homeostasis in E. coli by reducing the translation efficiency of Fur

To investigate the influence of iron and the ΔtusA and ΔmnmA deletion mutations on the overall protein abundance, a detailed proteomic analysis was performed. Cells of both strains and the corresponding BW25113 parental strain were cultivated in medium with KNO3 as electron acceptor and iron was removed from the medium by the addition of 150 M 2,2-DIP.