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Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1 2019/05/02

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https://zenodo.org/record/3234171
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Project: Biophysical investigation of purified HTT protein and protein complex samples Experiment: Coexpression and purification of HTT and HTT-HAP40 with NEDD4L and HAP1 Date completed:­ 2019/05/02 Rationale: HAP1 and NEDD4L have been suggested as high probability interaction partners of HTT although it is unclear if they interact with HTT or HTT-HAP40. Coexpression of the constructs was conducted in baculoviral expression system (Sf9 cells) to determine if they copurify with HTT or HTT-HAP40.
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2024-07-22
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