STEAP3 promotes TNBC growth through the FGFR1-mediated activation of PI3K/AKT signaling
收藏国家人口健康科学数据中心2026-06-01 收录
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https://www.ncmi.cn/phda/dataDetails.do?id=CSTR:17970.11.A001G.202406.136.V1.0
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资源简介：
Breast cancer cells were cultured and harvested for proteomic analysis. Cells were lysed using a buffer containing 8 M urea, and proteins were extracted by sonication. The lysate was centrifuged to remove debris, and the supernatant was collected. Protein concentration was determined using the BCA assay. Samples were then reduced with DTT, alkylated with IAA, and digested overnight with trypsin. Peptides were desalted using C18 columns before mass spectrometry analysis.
提供机构：
北京蛋白质组研究中心
创建时间：
2024-06-11
