Supplemental Material for Lustyk et al., 2019

Figure S1 shows male DMC1 hotspot activity in Hstx2 locus. Figure S2 contains examples of <i>Fmr1nb</i> expression at mRNA and protein level. Figure S3 shows domain structure of FMR1NB. Figure S4 shows comparison of litter size generated by B6.DX.1s and B6.DX.1s.<i>Fmr1nb^-</i> males. Figure S5 shows comparison of fertility parameters achieved by B6.<i>Fmr1nb</i>^- and B6.DX.1s.<i>Fmr1nb</i>^- males and their wild type counterparts. Figure S6 shows frequency of apoptotic cells in testes of B6.<i>Fmr1nb</i>^- and B6.DX.1s.<i>Fmr1nb</i>^- males. Table S1 contains microsatellite markers used for genotyping the X chromosome. Table S2 contain individual molecules report from DNA samples used for optical maps. Table S3 shows parameters of individual reference assemblies For optical mapping. Table S4 is composed of xx sub-tables each of them containing data source for indicated figures and tables. Table S5 contains Hstx2 candidate genes. Table S6 shows fertility parameters for hybrid males carrying either wild type or null mutant of <i>Fmr1nb</i> gene.