Diarrhea and virus shedding in Gn piglets after oral inoculation with VirHRV Wa (P[8]G1).

Treatment Group abbreviations as well as passive treatments administered to each group of pigs are clarified in Table 1. VHH = piglets treated with VHH nanoAbs to VP6 at a final Wa HRV ELISA Ab titer of 4096; IgY = piglets treated with IgY Abs to VP6 at a final Wa HRV ELISA Ab titer of 4096; CL = piglets treated with control larvae; IgG = piglets treated with porcine IgG Abs to Wa HRV at a final Wa HRV ELISA Ab titer of 4096; NT = not treated piglets; n = number of animals per group; AUC = area under the curve. ND = not detected. PID = post inoculation day.
aDetermined by ELISA and CCIF.
bDiarrhea duration was defined as the number of days with fecal score ≥2. Stool consistency was scored daily (0 = normal; 1 = pasty; 2 = semi-liquid; 3 = liquid).
cMean cumulative score was Σ(faecal scores ≥2)/number of animals. For statistical analysis, ND was considered as zero.
dProportions in the same column with different superscript upper case letters (A, B, C) differs significantly (Fisher's exact test, p<0.05).
eMeans in the same column with different superscript upper case letters differ significantly (Kruskall-Wallis rank sum test; p<0.05). Piglets were fed with 210 ml of sterile milk, twice a day, for 9 days with or without the addition of the corresponding volume of VP6 VHH pool, VP6 IgY pool, control larvae pool or Wa HRV porcine IgG pool, according to the experimental group.