Chromosomal rearrangement interferes with meiotic X-chromosome inactivation

Expression profiling of isolated populations of prepachytene spermatocytes, pachytene spermatocytes and spermatids of males carrying a translocation T(16;17)43H was performed to study the influence of this translocation on gene expression from individual chromosomes. To evaluate the transcriptional behavior of individual chromosomes in the sterile B10-T43/+ males and their fertile congenic B10-T43/T43 and B10 counterparts, we compared their transcriptomes in sorted populations of pre-pachytene primary spermatocytes, pachytene primary spermatocytes and spermatids. Keywords: individual genetic characteristics design Populations of pre-pachytene spermatocytes, pachytene spermatocytes and spermatids were isolated from three lines of mice: B10, B10-T43H/T43H and B10-T43H/+. All populations were isolated in duplicates from two animals. Hence, RNA was prepared from 18 samples and was then subject to labeling and hybridization on microarray chips.