scRNAseq analysis of kidney treated with GLS1 inhibitor [scRNA-seq Kid_BPTES]

We employed the Chromium GEM-X single-cell RNA sequencing platform by 10x Genomics to investigate the effects of the GLS1 inhibitor, BPTES, on the kidney single-cell transcriptome of 19- to 20-month-old wild-type male mice. Previous studies have identified GLS1 as a potential senolytic reagent capable of eliminating senescent cells. Our aim was to determine which cell populations are selectively removed by GLS1 inhibition in aged mice and to assess how this intervention alters intercellular interactions across different cell types. After administering BPTES or DMSO (vehicle control) to 19-month-old male mice for one month, we harvested the kidneys and dissociated the tissue using a Liberase-based enzymatic digestion protocol. The resulting single-cell suspension was then subjected to fluorescence-activated cell sorting (FACS) for cell collection prior to library preparation.