A 22-color Panel for the Measurement of Antigen-specific T-cell Responses in Human and Non-human Primates

The purpose of this work is to design a 22-color panel for the measurement of antigen-specific T-cell responses in human and non-human primates.
Conclusion:
This panel separates CD4+ T cells into Th1, Th2, Th17, and peripheral Tfh (pTfh) by measuring the expression of chemokine receptors (CXCR3, CCR4, CCR6, and CXCR5). Furthermore, it includes antibodies that detect an activation marker (CD154), a degranulation marker (CD107a), cytokines (IFNg, TNF, IL-2, IL-4, IL-13, IL-17, and IL-21), a chemokine (MIP1-b/CCL4), and immune checkpoint/activation molecules (PD-1 and ICOS).