Comparative DNA methylomic analyses reveal insight into potential origins of novel monocyte epigenetic biomarkers of insulin resistance

Genome wide DNA methylation profiling of monocytes from insulin sensitive and insulin resistant HIV-infected patients. The Illumina Infinium Human Methylation 450k Beadchip was used to obtain DNA methylation profiling across 450,000+ CpGs. Samples included monocytes from 37 HIV-infected individuals, of which 14 were insulin resistant and 23 were insulin sensitive, monocytes from 9 HIV-seronegative individuals, of which 4 were insulin sensitive and 5 insulin resistant, and FACS monocyte subsets (classical, intermediate, and non-classical monocytes) from two healthy donors. 500+ng of DNA was bisulfite converted from all 46 samples and hybridized to the Illumina Infinium Human Methylation 450K Beadchip