Micorscopy-based analysis chemical perturbation of gene-Pol II cluster visits in zebrafish embryos

Repository containing MatLab analysis scripts as well as intermediate analysis data files underlying the analysis of visits of oligopaint DNA FISH-labeled genes in zebrafish embryos in the stages oblong, sphere, dome, 30% epiboly, and 50% epiboly. The genes iscub, zgc::64022, and foxd5 were labeled in combination with immunofluorescnce staining against differently CTD-phosporylated RNA polymerase II (Pol II Ser5Phos, Pol II Ser2Phos). The image data were recorded on a VisiTech iSIM microscope.The image analysis code was partially generated and optimized using the artificial intelligence system Claude.The raw image data exceed a total size of 600 GB and are available upon request from the project leader.