Arabidopsis thaliana iP-delayed senescence transcriptome

It has long been known that cytokinins (including iP) delay senescence in detached leaves. Here, we performed RNA-seq analysis to evaluate possible mechanisms responsible for cytokinins delaying senescence. Cotyledons were excised from 12 day-old Arabidopsis thaliana seedlings and floated on MES buffer pH 5.7. Buffer was either supplemented with 1uM cytokinin or an equivalent volume of DMSO as a vehicle control. Cytokinins tested included iP as well as iP-7-glucoside and iP-9-glucoside. Treatment took place in the dark for six days to allow senescence to occur. RNA was extracted at the end of the six days.