Genome-wide analysis of gene expression in Panc-1 and BxPC-3 cells subjected to iExosomes treatment and control treatments

In this study Panc-1 cells and BxPC-3 cells were cultured. The cells were harvested (untreated control 'cont') for RNA extraction, or treated for 3 hours with various exosomes preparations. The exosomes were collected from BJ human foreskin fibroblast culture supernatant without further processing (control exosomes or 'CE'), or engineered to contain scrambled siRNA ('scr') or KRASG12D siRNA ('iExo). Two or three distinct wells of cells were evaluated per treatment condition and assigned a well number (well -1, -2 or 3). RNA from cultured Panc-1 and BxPC-3 human pancreas cancer cells was harvested. Controls included untreated cells, and cells treated with control exosomes and scramble siRNA containing exosomes. The exosomes were obtained from BJ human foreskin fibroblast culture supernatant. The recipient cells were incubated for 3 hours with the indicated treatments. The cells were also treated with iExosomes, which consist in exosomes obtained BJ human foreskin fibroblast culture supernatant and electroporated with siRNA that target KRASG12D.