Transcriptome sequencing with reference. Homo sapiens

In this analysis, 9 samples were sequenced, and a total of 62.80 G of CleanData was obtained. The effective data of each sample ranged from 6.86 g to 7.03 g, the Q30 bases ranged from 93.57% to 93.81%, and the average GC content was 51.40%. By aligning reads with the reference genome, the genome alignment of each sample was obtained, and the alignment rate was 98.05~98.52%. Based on the comparison results, the expression level of protein coding genes was analyzed. According to the expression level of protein coding genes in different samples, differential screening was conducted, and there were two differential groups, and the number of detected differential genes was 1722 and 1427 respectively.