Richard Allen (University of Hawaii), C. Schroeder (2012) CIL:40563, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

Quick-freeze deep-etch of acidosomes show the results of carrier vesicle fusion with the acidosomes. Both the E-face and P-face of the acidosomes appear to have two areas, the larger area on the E-face has few IMPs but has pits left by the removal of IMPs during fracturing and another area that is smooth with fewer pits that resembles a fused carrier vesicle membrane. A line marking the fusion site between these two vesicles is evident. The P-face of the acidosome has many IMPs while the fused carrier vesicle membrane is smooth. Presumably these two membrane areas will mix with time. Thus acidosomes, which are somewhat similar in appearance to multivesicular bodies in higher organisms, are late endosomes and deliver their membrane, their contents and their V-ATPase complexes to the phagosome to transform the phagosome to the phagoacidosome. TEM taken on 7/29/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 24,800X. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.

酸性小体的快速冷冻深蚀切片揭示了载体囊泡与酸性小体融合的结果。酸性小体的E面和P面均呈现出两个区域：E面上的较大区域含有较少的IMP，但在裂解过程中留下了IMP移除的坑洞；另一个区域则平滑且坑洞较少，类似于融合的载体囊泡膜。这两个囊泡之间的融合位点以一条标记线清晰可见。酸性小体的P面含有许多IMP，而融合的载体囊泡膜则平滑。推测这两个膜区域随着时间的推移将混合。因此，与高等生物中的多泡体在外观上有所相似的酸性小体，实际上是晚期内体，它们将膜、内容物和V-ATPase复合物传递至吞噬体，以将吞噬体转化为吞噬酸性小体。1988年7月29日，C. Schroeder使用Zeiss 10A在80kV下进行透射电子显微镜（TEM）拍摄。负片编号24,800X。经J. Cell Sci. 106卷第411-422页，1993年出版，已获许可改编。