Summary of qRT-PCR amplification of cartilage-selective genes in fetal cartilage and seven non-cartilage tissues.

Representative genes are listed in rank order of similarity to the human cartilage selective genes used in the seeding profile. Cart: mean Ct value for cartilage samples. NCart: Mean Ct value for the 7 non-cartilage samples. Neg: Number of non-cartilage tissues in which amplification was not detected at 35 cycles. Where no amplification was observed the maximum Ct value (i.e. 35) was used for calculations. Fold difference (Fold) is calculated from the difference in cartilage and non-cartilage Ct values.