Biological and environmental data for Di Cairano et al 2026

Samples of groundwater were collected from Bellevue Farm adjacent to the Namoi River in 2017-2018. Samples were analysed to characterise the water quality, prokaryote and eukaryote communities in the water. Water quality data were also collected. Biological assemblage data derived from Illumina sequencing of 16S rDNA and 18S rDNA amplicons. Sequence data were processed using Greenfield Hybrid Analysis Pipeline (GHAP) v2.2, created by CSIRO Australia (available at https://cloudstor.aarnet.edu.au/plus/s/ pUvwME6fMeCcX - zL). Taxonomic assignment is based on the Ribosomal Database Project (RDP) Classifier (Wang, Garrity et al. 2007). OTUs were assigned using a 97% similarity threshold). Low abundant OTUs (<10), organelles and unassigned taxa at kingdom level were removed.