Effect of autophagy modulation on ATG5 expression in DN ILC3s from NEC mice

xperimental Design Core Objective: Flow cytometry was performed to evaluate the effects of pharmacological autophagy modulation (inhibition by 3-Methyladenine (3MA), activation by Rapamycin) on ATG5 expression levels in intestinal double-negative (DN) group 3 innate lymphoid cells (ILC3s) from mice with necrotizing enterocolitis (NEC). Animal Model:Wild-type C57BL/6 mice were used in this study. All mice were subjected to standardized experimental NEC induction prior to pharmacological intervention and sample collection for flow cytometry detection.
Experimental Groups (3 groups total, fully matched to the figure): DMSO group: Vehicle control group (NEC-induced mice, treated with DMSO vehicle) 3MA group: Autophagy inhibition group (NEC-induced mice, treated with 3-Methyladenine (3MA), a classical autophagy inhibitor) Rapamycin group: Autophagy activation group (NEC-induced mice, treated with Rapamycin, a classical autophagy activator) Detection Targets (fully matched to the figure axes):Mean fluorescence intensity (MFI) of ATG5 in DN ILC3s (unit: ×10³), which reflects the expression level of ATG5 in DN ILC3 subsets isolated from the intestinal lamina propria.
Biological Replicates: 6 biological replicates (individual mice) per group Statistical Analysis: One-way ANOVA with Tukey’s post-hoc test was applied for multiple group comparisons. Statistical significance was defined as P < 0.05.