Transcriptome analysis of AAV-induced retinopathy models expressing human VEGF, TNF-a and IL-6 in murine eyes

High-throughput sequencing of murine retina and RPE/Choroid (eye cup) tissue following mueller-cell mediated expression of either hVEGFA, hTNFa, or hIl6 by adeno-associated viruses (AAV) Overall design: We intravitreally injected mouse eyes with AAVs expressing one of the three human transgenes VEGF, TNFa, or IL6. As controls, we used AAV-stuffer injected eyes at matching concentrations and non-injected eyes. From all treatment groups total RNA was extracted from the retina and the posterior eye cup, including retinal pigment epithelium (RPE), choroid, sclera and the ciliary body. Sample collection was followed by RNA sequencing.