RIFT VALLEY FEVER VIRUS TYPING TOOL

Therefore, we have developed a computational tool for rapidly classifying and assigning lineages of the RVFV isolates. The computational method is presented both as a command line tool and a web application hosted at https://www.genomedetective.com/app/typingtool/rvfv/ . Validation of the tool has been performed on a large dataset using glycoprotein gene (Gn) and whole genome sequences of the Large (L), Medium (M) and Small (S) segments of the RVFV retrieved from the National Center for Biotechnology Information (NCBI) GenBank database. Using the Gn nucleotide sequences, the RVFV typing tool was able to correctly classify all 234 RVFV sequences at species level with 100% specificity, sensitivity and accuracy.

据此，我们开发了一款可快速对裂谷热病毒（Rift Valley Fever Virus, RVFV）分离株进行分类并确定其谱系的计算工具。该计算方法同时以命令行工具与网页应用的形式对外提供，部署于网址https://www.genomedetective.com/app/typingtool/rvfv/。本工具已基于大规模数据集完成验证，所用序列为从美国国家生物技术信息中心（NCBI）GenBank数据库中获取的RVFV糖蛋白基因（Gn）以及大（L）、中（M）、小（S）基因节段的全基因组序列。利用Gn核苷酸序列，该RVFV分型工具可在物种水平上对全部234条RVFV序列实现精准分类，其特异性、灵敏度与准确率均达到100%。

搜集汇总

背景与挑战

背景概述

该数据集提供一种用于快速分类和分配裂谷热病毒分离株谱系的工具，支持命令行和网页应用。工具通过Blast和系统发育方法，基于M段基因进行基因分型，已验证在大型数据集上具有高准确性和特异性。