ASSEMBLY: Metaviromes Reveal the Dynamics of Pseudomonas Host-Specific Phages Cultured and Uncultured by Plaque Assay

Dataset description This record contains 12 metavirome assembly FASTA files associated with the article “Metaviromes Reveal the Dynamics of Pseudomonas Host-Specific Phages Cultured and Uncultured by Plaque Assay.” The assemblies were generated from an organic-waste-derived virome used to study phages associated with Pseudomonas syringae pv. tomato DC3000. The dataset includes baseline, DC3000-spiked, non-spiked control, pooled, hybrid, and long-read-supported assemblies: A01_baseline_metaSPAdes.fasta: baseline virome assembly generated with metaSPAdes from Illumina short reads. A02_DC3000_p_T_metaSPAdes.fasta: pooled/total assembly of the DC3000-spiked virome replicates generated with metaSPAdes. A03_DC3000_p_A_metaSPAdes.fasta: DC3000-spiked virome replicate A assembly. A04_DC3000_p_B_metaSPAdes.fasta: DC3000-spiked virome replicate B assembly. A05_DC3000_p_C_metaSPAdes.fasta: DC3000-spiked virome replicate C assembly. A06_DC3000_n_T_metaSPAdes.fasta: pooled/total assembly of the non-spiked control virome replicates generated with metaSPAdes. A07_DC3000_n_A_metaSPAdes.fasta: non-spiked control replicate A assembly. A08_DC3000_n_B_metaSPAdes.fasta: non-spiked control replicate B assembly. A09_DC3000_n_C_metaSPAdes.fasta: non-spiked control replicate C assembly. A10_all_hybrid_metaSPAdes.fasta: hybrid assembly generated from combined virome reads using short-read and long-read information. A11_baseline_polished_all_Canu.fasta: baseline virome long-read assembly generated with Canu and polished using reads from all virome samples. A12_baseline_polished_baseline_Canu.fasta: baseline virome long-read assembly generated with Canu and polished using only baseline virome reads. Together, these assemblies allow comparison of viral contigs recovered from the original virome, host-exposed viromes, control viromes, and different assembly strategies. They support analyses of viral community composition, Pseudomonas-associated phage dynamics, and comparison between cultured plaque-isolated phages and uncultured phages detected by metaviromics. Recommended citation Alanin, K. W. S., Junco, L. M. F., Jørgensen, J. B., Nielsen, T. K., Rasmussen, M. A., Kot, W., & Hansen, L. H. (2021). Metaviromes Reveal the Dynamics of Pseudomonas Host-Specific Phages Cultured and Uncultured by Plaque Assay. Viruses, 13(6), 959. https://doi.org/10.3390/v13060959.