BRED frequencies for constructing deletions in the tape measure gene of mycobacteriophage Giles.

1Mutants generated have in-frame deletions in Giles gene 20; deletion sizes in base pairs (bp).
2Substrates were either oligonucleotides (name-length) or dsDNA as indicated.
3Plaques recovered from electroporation analyzed by PCR as indicated; NT, not tested.
4Substrate also incorporates point mutations; frequencies reflect deletion formation only.