Hierarchical deployment of Tbx3 dictating the identity of hypothalamic Tac2/Kiss1 neurons

To investigate how Tbx3 regulates the fate determination of arcuate piptidergic neruons, we performed scRNA-seq, snRNA-seq and CUT&Tag to reveal the function of Tbx3 in fate specification and maintenance of neurons Lineage tracing experiment: scRNA-seq of Tbx3-CreER::Ai14 mice (E9 > P14); Tbx3 knockout experiment: scRNA-seq and snRNA-seq of Nkx2.1-Cre::Tbx3 (Flox/Flox) mice and its littermate control (P14); CUT&Tag experiment: Nkx2.1-Cre::Tbx3 (Flox/Flox) mice and its littermate control (P0) Please note that NKO mice is a conditional knockout mice strain that lacking the expression of Tbx3 in hypothalamus and, like the input sample, peak-calling was performed based on reads of the blank control (NKO mice) in the experiment of CUT&Tag. Therefore no processed data was provided for both 'NKO mice_CUT&Tag_WT_input' and 'NKO mice_CUT&Tag_NKO_Ab' samples.