Visium spatial transcriptomics analysis of lacrimal gland during chronic inflammation progression.

To identify the transcriptomic alterations within the different cellular compartments of the lacrimal gland during chronic inflammation, we analyzed the lacrimal glands of NOD.B10.H2b vs BALB/cJ with 10X Visium technology We sequenced RNA from lacrimal glands cryosections (10m) of BALB/cJ (control) and NOD.H2b (disease) mice aged of 4 months in duplicates.为解析慢性炎症过程中泪腺（lacrimal gland）不同细胞区室内的转录组改变，本研究采用10X Visium技术对NOD.B10.H2b与BALB/cJ小鼠的泪腺开展分析。我们对4月龄BALB/cJ（对照组）及NOD.H2b（疾病模型组）小鼠的泪腺冰冻切片（厚度10微米）进行RNA测序，实验设置平行重复。