Additional file 1 of Water channel protein AQP1 in cytoplasm is a critical factor in breast cancer local invasion

Additional file 1: Supplementary Fig. 1. Cytoplasmic expression of AQP1 was positively correlated with breast cancer progression. (a) Patients who had a recurrence or metastasis had a higher AQP1 cytoplasmic expression (62.2% vs 38.9%, P = 0.009). Cyto-AQP1: cytoplasmic AQP1 expression. (b) Patients who had a lymph node metastasis (n > 4) had a higher AQP1 cytoplasmic expression (50.0% vs 38.1%, P = 0.038). Cyto-AQP1: cytoplasmic AQP1 expression. (c-d) The relationship between pT stage and AQP1 cytoplasmic expression. Cyto-AQP1: cytoplasmic AQP1 expression. (e) The tumor volume in Flag-vector/MDA-MB-231 and Flag-AQP1/MDA-MB-231 mice group. Values were expressed as mean ± SD (two-tailed Student’s t test and two-way ANOVA, **P 60 cells per group, two-tailed Student’s t test). Scale bar=20 μm. All experiments were independently repeated for three times. Supplementary Fig. 9. The effect of the expression of AQP1/ANXA2/Rab1b/CTSS/ICAM1 on the prognosis of breast cancer patients. (a) Kaplan–Meier analysis showed the association between AQP1 expression and overall survival of breast cancer patients in GEPIA database (log-rank test). (b) Kaplan–Meier analysis showed the association between ANXA2 expression and overall survival of breast cancer patients in Kaplan-Meier plotter database (log-rank test). (c) Kaplan–Meier analysis showed the association between Rab1b expression and overall survival of breast cancer patients in Kaplan-Meier plotter database (log-rank test). (d) Kaplan–Meier analysis showed the association between CTSS expression and overall survival of breast cancer patients in Kaplan-Meier plotter database (log-rank test). (e) Kaplan–Meier analysis showed the association between ICAM1 expression and overall survival of breast cancer patients in Kaplan-Meier plotter database (log-rank test). Supplementary Fig. 10. Relationship of AQP1 with ANXA2, Rab1b, CTSS and ICAM1. (a) In 194 IDC patients, AQP1 had a positive correlation with ANXA2. (b-d) Correlation analysis using GEPIA (http://gepia.cancer-pku.cn) indicated that AQP1 was positively correlated with Rab1b, CTSS and ICAM1 in breast cancer patients.