Cellular response to small molecules that selectively stall protein synthesis by the ribosome. Cellular response to small molecules that selectively stall protein synthesis by the ribosome

We use ribosome profiling to compare the selectivity of two small molecules, PF-06446846 and PF-06378503 that inhibits translation of their target by selectively inducing ribosome-stalling in a nascent chain sequence dependent manner. Overall design: Huh7 cells were treated with 1.5 µM PF-06446846, 1.5µM PF-06378503 or vehicle for 60 minutes, and subjected to ribosome profiling. This study included three biological replicates